ROOTS Ranking
(Groupe A)
|
||||
NAA→ ;
Kinetin ↓
|
0ml
|
0.03ml
|
0.3ml
|
3ml
|
0ml
|
4
|
4
|
8
|
9
|
0.03ml
|
3
|
3
|
7
|
8
|
0.3ml
|
2
|
2
|
7
|
8
|
3ml
|
1
|
2
|
5
|
7
|
ROOTS Ranking
(Group B)
|
||||
IBA→ ; BAP ↓
|
0ml
|
0.03ml
|
0.3ml
|
3ml
|
0ml
|
6
|
6
|
9
|
9
|
0.03ml
|
7
|
7
|
7
|
9
|
0.3ml
|
6
|
3
|
3
|
3
|
3ml
|
3
|
3
|
3
|
3
|
Group A
worked with Saintpaulia sp. and the hormones NAA und Kinetin and
Group B worked with
Chrysanthemum plants and the hormones IBA and BAP.
The tables show the
different combination of hormones and the effects on the root and callus
formation. We gave the root/callus formation a ranking from 1 (worst) to 9
(best) with regard to further propagation. A 9 would be many short, sick roots
and a 1 would be brown tumour without any roots.
The results of the two groups where quite
different. It is also hard to compare the two cultures, because Saintpaulia sp.
forms rosettes and Chrysanthemum grows tall with long shoots.
In general we can say
that it is better to put more auxin than cytokinine. The best results in both
hormone squares where the ones with 3ml auxin and 0ml cytokinine (box number 4,
compare box numbers with previous blog). Both groups had the worst results with
3ml cytocinine and no auxin added. Everything between is a bit of a mix of
rankings from 1 to 9. However, this is just with regard to the rooting.
On boundless (2013)
I found an article about what we should expect with different auxin and
cytokinine combinations: "When cytokinin and auxin concentrations are approximately equal in a plant, plant cells do
not differentiate. If only auxin is
present, plant cells will enlarge, but will not divide. If only cytokinins are
present, no changes occur in the plant cells. If cytokinins are present at
higher concentrations than auxin,
both cell enlargement and
division will occur. Increase in auxin levels causes root formation."
Most of these effects are
already visible on our cultures and the others might just need a little more
time. On the other hand, we had already effects of just cytokinine added and no
auxin.
References:
BOUNDLESS. (2013). Cytokinins Control Cell Growth and Differentiation
. Abgerufen am 06. Mai 2013: www.boundless.com
